Krystal hope nusbaum biography of michael

Abstract

An unmet need in cell engineering is the availability of a single transgene encoded, functionally inert, human polypeptide that can serve multiple purposes, including ex vivo cell selection, in vivo cell tracking, and as a target for in vivo cell ablation. Here we describe a truncated human EGFR polypeptide (huEGFRt) that is devoid of extracellular N-terminal ligand binding domains and intracellular receptor tyrosine kinase activity but retains the native amino acid sequence, type I transmembrane cell surface localization, and a conformationally intact binding epitope for pharmaceutical-grade anti-EGFR monoclonal antibody, cetuximab (Erbitux). After lentiviral transduction of human T cells with vectors that coordinately express tumor-specific chimeric antigen receptors and huEGFRt, we show that huEGFRt serves as a highly efficient selection epitope for chimeric antigen receptor T cells using biotinylated cetuximab in conjunction with current good manufacturing practices (cGMP)-grade anti-biotin immunomagnetic microbeads. Moreover, huEGFRt provides a cell surface marker for in vivo tracking of adoptively transferred T cells using both flow cytometry and immunohistochemistry, and a target for cetuximab-mediated antibody-dependent cellular cytotoxicity and in vivo elimination. The versatility of huEGFRt and the availability of pharmaceutical-grade reagents for its clinical application denote huEGFRt as a significant new tool for cellular engineering.

Introduction

Cell-based therapies, including genetically manipulated cell products, are an emerging area in applied biotechnology. Foremost in the cell therapy field, and now a component of standard medical practice, is hematopoietic stem cell transplantation. Likewise, adoptive transfer of T cells for infectious and neoplastic disease is the subject of intense clinical research. In both of these cell therapy applications, a variety of genetic engineering approaches are being studied to endow hematopoieti

Logan Rohrkemper, 40, of West Hurley, NY, passed away on Thursday, July 1, at her home in West Hurley, NY, surrounded by her loving family.
She was born on October 22, in San Luis Obispo, CA, and was the daughter of Anthony and Beth Rohrkemper. Her family moved to Three Rivers, CA in where she attended Three Rivers Union School and Exeter High School.

After graduating High School, Logan attended community college in Visalia, CA, then eventually moved to Sacramento, CA, where she worked and studied art in the Art Institute. She then moved to Seattle, WA, where she worked as a nanny for 8 years while continuing with her art. She worked mainly in acrylic paint and water color and her work was shown in several local establishments around Seattle. Her whimsical pet portraits were particularly popular, and her skill at capturing the heart of the local characters was incomparable.

Logan loved music of all genres and especially loved attending live concerts. She was blessed to have seen many of her musical heroes live and provided great reviews on all. Her infectious laugh and adventurous spirit was shared with her many friends who have lots of those memories to hold dear.

She is survived by her dear Persian kitty Mildred, her mother, Beth Rohrkemper, her maternal grandmother Harriette Schmidt; her loving uncles Al Nussbaum; Bill Nussbaum (Marilyn Messa); Aunt Anne (Bill) Turnbull; Unlcle Mike (Gerry) Rohrkemper; as well as niece Ema Turnbull, nephews Kazuma Turnbull, Connor and Ian Axup; cousins Sarah (Jared) Axup, and Kevin (Chiemi) Turnbull, many more cousins and hundreds of friends.

Logan is preceded in death by her father, Anthony Rohrkemper and paternal grandparents, Ralph and Kate Rohrkemper, her uncle Jim Rohrkemper, and her maternal step grandfather, John Schmidt.

Arrangements entrusted to Keyser Funeral & Cremation Service, Inc., Albany Avenue, Kingston, NY A tribute for Logan may be found at , where you may leav

Photo by Sara Jane Inkster

As an essential part of the 60s British New Wave Sci-Fi movement, Michael Butterworth's earliest work helped define this "new writing" in the groundbreaking New Worlds Magazine, taken over in by Michael Moorcock to foster this pivotal sea change. After many of these short pieces would be compiled in his collection Butterworth, he'd author his first novel, My Servant The Wind, before writing the Hawklords series of fantasy paperbacks based on the rock group Hawkwind. Butterworth would eventually start Savoy Books, a bookshop and publishing house in Manchester where he'd release the controversial Lord Horror novel by David Britton, which he also contributed text to and edited. The book triggered one of the longest, most truly fascist examples of "cancellation" our society might ever see, and makes our current state of knee-jerk culture look crybaby in comparison. 

But far beyond his experimental, prescient glimpses into future and revolutionary tales of the past, Butterworth is a poet at heart. This week Space Cowboy Books releases his Complete Poems , which chronicles his dynamic life through verse. A book with an accompanying album of guest musicians backing up his reading of selected pieces, Complete Poems is a hybrid document of an exceptional man's journey, one Alan Moore (The Watchmen, From Hell) calls "rolling news from a since-outlawed territory of ideas; bulletins filed from a redacted country, edited out of cultural continuity human moments are examined as though artifacts dug from the future, or the debris of a missing world. Caught in a jeweler’s eyepiece, fugitive impressions from near sixty years of subterranean endeavor here condense to lyric crystal, ringing with the poet’s radical and laser-guided voice."

This new poetry volume is much more than just a collection—it’s an artful memoir of sorts, going beyond the mere chronicle